Research plan for the Panther Chameleon Furcifer pardalis in Madagascar January-February 2009.

 

BACKGROUND

The panther chameleon Furcifer pardalis is one of the more spectacular natural treasures that one is likely to encounter while touring the agricultural countryside and villages of coastal northern Madagascar . These spectacular animals are large (males up to 18 or more inches in length), brightly colored, and conspicuously active while climbing among eye-level vegetation.

Over the past few decades much has been learned about their natural history. Adult panther chameleons breed during the warm, rainy, summer months, females producing from one to several clutches of one- to two-dozen eggs buried deep in the ground. After a long (up to a year) incubation period the hatchlings emerge, grow rapidly and mature at as early an age as 6 months, although males may take up to a year to reach full size. While males can exceed five years of age in captivity, most wild panther chameleons probably survive only one or two years after maturity. They feed mostly on terrestrial invertebrates but have been observed to eat small vertebrates, including juveniles of their own species, and, rarely, plant material. On sunny days they behaviorally adjust their body temperature to about 30 degrees C, but on overcast days they carry out their activities with a body temperature that closely matches that of their surroundings. During the breeding season males are strongly territorial and will threaten an intruding male with open-mouth posturing, color change (in some populations) and chase followed by attack, if necessary. Staging an encounter makes for spectacular photo-opportunities for eco-tourists, but one must take care not to allow combat which can result in severe injury or death to the combatants. Using a mirror to simulate intrusion and elicit display sometimes works just as effectively.

While panther chameleons are hardy captives, they were initially difficult to breed successfully in captivity due to poor ultraviolet light environments for females. Ultraviolet (UV) light from the sun is known to produce vitamin D in the skin of many vertebrates including ourselves and panther chameleons. UV-induced vitamin D serves as a hormone in the body to help regulate calcium-phosphorus balance in the body. Its chief function is to stimulate the uptake of calcium from the gut and inhibit calcium re-absorption from bone. In short without adequate vitamin D from either UV exposure or dietary intake females cannot provide enough vitamin D to their eggs to enable the developing embryos to form their skeletons. Their eggs fail to hatch.

An important discovery made in the last few years was that females seem to be able to sense their internal vitamin D-condition, perceive an external UV source and voluntarily expose themselves to that source when they are vitamin D deficient. Alternatively, they choose to avoid high UV sources when they are vitamin D sufficient. In short they seem able to “photo-regulate” their vitamin D condition much like many lizards thermoregulate their body temperature by shuttling between heat sources and heat sinks in their environment. Prior to our studies no vertebrate had been shown to possess this “vitamin D-UV photo-regulation” ability. Certainly we don’t! Our most recent research has shown that other lizard species besides the panther chameleon may possess this same ability, so it may be an adaptive mechanism for lizards in general.

All of the research on panther chameleons leading to the discovery outlined above has been conducted in the laboratory. There are missing pieces of information regarding the UV environment of panther chameleons in nature that can be rapidly filled in a few days by an eco-tour group and will increase our understanding of this phenomenon. We know that the UV requirement of reproducing adult females and juveniles is greater than that of adult males in captivity. Are the UV environments of adult breeding females and juveniles higher than those of adult males in nature? We know what the optimum dose of UV is for breeding females in captivity to ensure good hatching success. Because females in captivity seem willing and able to expose themselves to the amount of UV necessary, we need to see if UV exposures in the field are similar to those predicted from our laboratory studies. Something that we have not learned either in captivity or in nature is how females balance their need for UV exposure with their need for thermal exposure. The sun produces both heat (infrared radiation or IR) and UV. In open habitats these two irradiances are highly correlated, i.e., bright sunny spots are high in all light irradiances, including IR and UV. However in filtered sunlight IR and UV radiation become somewhat dissociated. Must females and juveniles with their higher UV requirements tolerate higher body temperatures than adult males or can they dissociate these to the extent that they can obtain higher UV exposure but maintain the same body temperature? With some simple observations and measurements in the field we can answer these questions.

 

PROCEDURES

Our objectives will be to search the vegetation for chameleons between the hours of 0800 and 1700. While our focus will be panther chameleons, data for any species will be valuable. For every animal encountered we will record the following data in the following sequence:

1. species, sex, and age category, e.g. “adult male panther chameleon”

2. subjective assessment of sun exposure when spotted (full sun, partial sun, shade). Noticeably darkened coloration of the animal should be recorded.

3. time and cloud-cover conditions when spotted (sunny, filtered sun, overcast)

4. activity when spotted (sitting, moving, feeding, courting, displaying to same-sex individual etc.)

5. flank temperature (both sides of animal ) taken using a remote-sensing laser temperature gun.

6. UVB exposure where first spotted using Solartech 6.2 and 6.5 UVB meter (three readings- vertical, horizontal, pointing toward sun)

7. visible light exposure where first spotted using light meter (same three readings as for UVB)

     *** steps 1-7 should be completed in less than 3 minutes, if possible***

8. at this point animal can be photographed, observed manipulated (e.g. fed, shown mirror etc.)

9. SVL, mass, cloacal temperature. Animal will be captured, measured with a meter stick or ruler and weighed with a Pesola spring scale. Cloacal temperature will be taken with a Miller-Weber quick-reading thermometer but only if step 8 is minimized and/ or there is no reason to think that the animal has substantially changed its thermal environment.

10. animal will be released at site of capture or retained for other purposes.

11. Ideally, at least two crews working independently will be formed and work simultaneously.

 

Gary Ferguson, June 2008, and his best-known panther chameleon books

 

Professional Biographical Sketch of Gary W. Ferguson

a. Professional Preparation.

            Tulane University   Zoology  Bachelor of Science 1963

            Texas Tech University Biology Master of Science 1965 

            University of Michigan Zoology Doctor of Philosophy 1969

 

b.  Appointments.

            Professor Emeritus, 2007 to present

            Professor, Texas Christian University , 1983 to 2007

            Associate Professor, Texas Christian University , 1977 to 1983

            Assistant Professor, Texas Christian University , 1974-77

            Assistant Professor, Kansas State University, 1969-74.

 

c. Selected Publications (92 total since 1965).          

 

Lindgren,J, W.H.Gehrmann, G.W.Ferguson, and J.E.Pinder 2008. Measuring effective vitamin D3-producing ultraviolet B radiation Using Solartech's Solarmeter 6.4 Handheld, UVB Radiometer. Bulletin of the Chicago Herpetological Society 43:57-62.

 

Ferguson , G.W. J. B. Murphy, A. Raselimanana, J. Ramanamanjato. (2007). Panther Chameleon (Chamaeleo pardalis):Natural History, Captive Management and Breeding. Chapter in Captive Care of Veiled, Jackson ’s Panther, and Parson’s Chameleon, Bowtie Press.

 

 Ferguson, G.W., W.H. Gehrmann, K.B. Karsten, A.J. Landwer, E.N. Carman, T.C. Chen, M.F. Holick. (2005. Ultraviolet exposure and vitamin D synthesis in a sun-dwelling and shade-dwelling species of Anolis: are there adaptations for lower UVB and vitamin D3 availability in the shade? Physiological and Biochemical Zoology 78:193-200.

 

Ferguson , G. W., J.B. Murphy, J. Ramanamanjato, A.P. Raselimanana. 2004. The panther chameleon: color variation, natural history, conservation and captive management. Krieger Publishing Co. Malabar FL.

 

            Ferguson, G.W., W.H. Gehrmann, K.B. Karsten, S.H. Hammack, M. McRae, T.C. Chen, N.P. Lung, M.F. Holick. 2003. Do panther chameleons bask to regulate endogenous vitamin D3 production? Physiological and Biochemical Zoology 76:52-59.

 

Ferguson G.W., W.H. Gehrmann, T.C. Chen, E.S. Dierenfeld, M.F. Holick. 2002. Effects of artificial ultraviolet light exposure on reproductive success of the female panther chameleon (Furcifer pardalis). Zoo Biology 21:525-537.

 

Carman, E.N., G.W. Ferguson, W.H. Gehrmann, T. Chen, M.F. Holick. 2000. Photobiosynthetic opportunity and ability for UV-B generated vitamin D synthesis in free-living house geckos (Hemidactylus turcicus) and Texas spiny lizards (Sceloporus olivaceous). Copeia 2000:245-250.

 

            Ferguson, G. W., J.R. Jones, W.H. Gehrmann, S.H. Hammack, L.G. Talent, R.D. Hudson, E.S. Dierenfeld, M.P. Fitzpatrick, F.L. Frye, M.F. Holick, T.C. Chen, Z. Lu, T.S. Gross, J.J. Vogel. 1996.  Indoor husbandry of the panther chameleon Chamaeleo (Furcifer) pardalis: effects of dietary vitamins A and D and ultraviolet irradiation on pathology and life-history traits. Zoo Biology 15:279-299.

 

            Ferguson , G. W. 1994. Old-world chameleons in captivity: growth, maturity and reproduction of panther chameleons (Chamaeleo pardalis) from Madagascar . Pp.323-331. In J. Murphy, K. Adler, and J.T. Collins (eds). Captive Management and Conservation and Captive breeding of Reptiles and Amphibians. Society for the Study of Amphibians and Reptiles, Ithaca , New York . Contributions to Herpetology vol. 11.

               

Ferguson ,  G.W.  and  L.G.  Talent. 1993. Life-history traits of the lizard Sceloporus undulatus from two populations raised in a common laboratory environment. Oecologia 93: 88-94.

 

              Ferguson , G.W. and S.F. Fox.  1984.  annual variation of survival advantage of large juvenile side-blotched lizards, Uta stansburiana:  its causes and evolutionary significance.  Evolution 38:342-349.

 

              Ferguson , G.W., J.L. Hughes, K.L. Brown.  1983.  Food availability and territorial establishment of juvenile Sceloporus undulatus.  Pp. 134-148. In R.B. Huey, E. Pianka, T. Schoener (eds.)  Lizard Ecology:  studies of a model organism.  Harvard University Press. Cambridge , MA .

 

              Ferguson , G.W., C.H. Bohlen and H.P. Woolley.  1980.  Sceloporus undulatus:  comparative life history and regulation of a Kansas population.  Ecology 61(2):313-322.

 

              Ferguson , G.W.  1977.  Variation and evolution of stereotyped behavior in reptiles, Part II, social displays of reptiles.  Pp. 405-457. In Gans and Tinkle, (eds).  Biology of the Reptilia, Vol. 7. Academic Press. New York , NY .

 

Ferguson , G.W.  1971.  Variation and Evolution of the push-up displays of the side blotched lizard Genus Uta (Iguanidae).  Systematic Zoology 20:79-101.

 

 

d. Professional activities.

            Invited Speaker, Symposium on the Biologic Effects of Light, Basil Switzerland, November, 1998; Editorial Board, Copeia; American Society of Ichthyologists and Herpetologists, Chairman, Local Committee 60th Annual Meeting held at TCU in 1980; Society for the Study of Reptiles and Amphibians, President (nominee); National Geographic Society and British Broadcasting Corporation, Technical consultant for nature films production. Lincoln Park Zoo, Invited workshop participant, Conference on Vitamin D, April 2003. Dean’s award for outstanding research achievement ( Texas Christian University ).

 

e.  Collaboration and other Affiliations

         (i) Collaborators

                Cynthia Bennett, Dallas Zoo; Elliott Carman, Allan Plummer Associates; Tai Chen, Boston University; Ellen Dierenfeld, New York Zoological Society; William Gehrmann, Texas Christian University; Stephen Hammack, Fort Worth Zoo; Michael Holick, Boston University; Amy Hunt, Fort Worth Zoo; Kris Karsten, Oklahoma State University; Allan Landwer, Hardin Simmons University; Nancy Lung, Fort Worth Zoo; Matthew Russell, Dallas Zoo; John Pinder, Colorado State University; Chris Raxworthy, American Museum of Natural History; Stephen Tidd, Boots and Coots Special Services; Ann Ward, Fort Worth Zoo; Brian Aucone, Oklahoma City Zoo.

         (ii) Graduate Advisor

                Dr. Donald W. Tinkle, The University of Michigan, deceased

         (iii) Thesis Advisor

                I have advised 24 graduate students, mostly Master of Science students, since 1965. Those since 1995 are: Jon Jones, affiliation unknown; Steven Tidd, Boots and Coots Services; Elliott Carman, Allan Plummer Associates; Jennifer Fontana, Trinity Valley School, Fort Worth; Kris Karsten, Oklahoma State University; Kelly Rypkema, Heard Museum: Christine Bertz, current graduate program, The University of Mississippi, Andrew Brinker, Faculty, Paschal High School, Fort Worth, Stacey Bucklin, Buxton Marketing Corporation, Fort Worth.